Major consumers of common bean in these countries often live on less than two U.S. dollars per day, where beans are grown primarily by smallholder farmers on less than two hectares (McClean and Raatz 2017). Recently, multi-trait mixed models (MTMM) statistical methods have been developed to uncover common genetic effects that act in a pleiotropic manner on two correlated traits (Korte et al. The best model was chosen based on the lowest calculated MSD value (Mamidi et al. Days to flower in Honduras, (Trait 1) and Puerto Rico (Trait 2) grown in 2016. For DTF, this correlation was high (r = 0.96) and very significant (Table 1), and without environmental effects. This is a direct advantage for a project with more limited resources because statistically sound results can reveal important genetic relationships that would not have been detected with a MLM analysis with smaller panel sizes. B. ML phylogenetic tree of BASE genotypes. The phenotypic variation explained by a significant marker was described as a likelihood-ratio-based R2 (R2LR; Sun et al. BWA-MEM (Li 2013), and Samtools (Li et al. GWAS were performed for each trait in each location under different stress conditions using untransformed data. A genetic discovery population carefully designed to include variation for response to heat and/or drought stress is important for discovering critical genetic factors associated with the abiotic stress response. The scatter plot region can be easily changed by input a new region and 'SEARCH' or click on a BIN in the navigational Manhattan Plot panel. 1991; Schmutz et al. Consistent with the individual trials, the peak SNP accounted for 8.4% of the variation (Table S2). Genet Epidemiol. These HapMaps are a major output from the USAID CRIB project and will be an important genetic resource well beyond the end of the project.  |  Days to flower GWAS results for the panel grown under heat in Honduras and Puerto Rico in 2016. The peak SNP for yield (Pv03:41,096,424 bp; P = 9.05E-8) is located on the distal end of chromosome Pv03 and explains 14% of the variation in yield (Table S2). Middle American genotypes = red and green; Andean genotypes = purple and blue. ROS regulation during abiotic stress responses in crop plants. In UKB, the sample overlap in the summary statistics across the traits is known, whereas in 23andMe, the sample overlap in the summary statistics is unknown. (F Joint heat and drought SPAD reading GWAS analysis. On Pv11, significant peaks were observed at 4.0 Mb and 45.3 Mb. Candidate genes were selected from an interval that ranged from 50kb upstream to 50kb downstream of the peak SNP using the common bean v2.1 gene models ( Phylogenetic analyses demonstrated the BASE populations represented broad genetic diversity for the appropriate races within the two gene pools. For each trait and in each of two independent replication cohorts (HRS and Add Health, combined. Neurobiol Stress. From a plant breeding perspective, the development of molecular markers that are functional across locations should be possible. As mentioned above, both Nacome, HN and Juana Diaz, PR are high heat stress environments. If not specified, the most significant region of all selected traits was displayed. Sequencing barcodes were removed and low-quality sequences were trimmed. Genomics, genetics and breeding of common bean in Africa: A review of tropical legume project. Whereas the BASE_120 and BASE_Meso consist primarily of cultivars and advanced breeding lines, the BASE-Andean contains a large portion of landraces from East Africa. MLM GWAS analysis for BASE panels. Genome-wide linkage and association mapping of halo blight resistance in common bean to race 6 of the globally important bacterial pathogen. Therefore, there is a real need to improve common bean productivity in these regions for fast growing populations that will be affected by anticipating future conditions brought on by climate change (Beebe et al. These locations can represent similar environments such as regional crop production sites that experience somewhat similar weather patterns or diverse sites that cross national or continental boundaries. The peak SNP was located at 40,504,942 bp (P = 9.02E-06) and accounted for 9.9% of the variation. 2011). 1. Quantitative trait loci associated with resistance to Empoasca in common bean. For the full MTMM model, eight genomic regions passed the –log10(P) > 5.0 threshold. Methods for meta-analysis of multiple traits using GWAS summary statistics. Combining the single-trait GWAS in a multi-trait analysis resulted in 563 and 263 significant SNPs at significance thresholds of P < 10 −5 and P < 5 × 10 −7, respectively. We do not retain these email addresses. (2012). 7. The snpEff database was used to describe potential effects of SNPs within the ±50kb interval of a peak SNP. Maximizing the number of SNPs within any collection of genotypes will increase the likelihood of finding associations with a target phenotype in the full collection or a subset of the genotypes. Table S1 contains the list of BASE genotype names. The experimental lines were planted on raised 1.5 m wide beds with two rows spaced 0.6 m apart. 2020 Oct 19;16(10):e1009089. A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. The range of SNPs per Mb is consistent across both and heterochromatic of the and Andean SNP data sets (Table S3). Sl-IAA27 gene expression is induced during arbuscular mycorrhizal symbiosis in tomato and in Medicago truncatula. This is encouraging for marker assisted breeding because only a single or a few markers may be needed for selection for days to flower in these two heat stress environments. There were no significant loci for single-trait BH6 (sin-gle-trait GWAS for BH at 6months after birth, and so forth), single-trait AS6, single-trait CS6, and LONG-AS. The data from 23andMe for SWB are newly analyzed data for this paper. Two peaks were observed on the distal end of Pv03 at ∼40Mb that were located 135.2 kb apart. This tree was developed with the 5,637 SNPs shared between the MA and Andean SNP data sets. Because of resource constraints for field research in these target regions, the panels were designed to be modest in size (n∼120 lines). Figure 3C shows that selection for the early DTF allele will have a positive effect on yield performance under high heat conditions. The MTMM analysis of DTF data from the BASE_Meso population grown in HN and PR under heat in 2016 showed the full joint analysis out-performed individual marginal analyses (Figure S2). National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error, Collaborators, In the latter case, this interaction reflects the genotype x environment interaction effect that is important in the context of breeding for multiple environments. These two traits had a very high and significant genetic correlation (r = 0.90) that lacked an environmental correlation. From the perspective of developing association panels, the unique LD structure within the two bean gene pools and the repeated observation that phenotypes are often controlled by different genetic factors in the two pools makes it imperative that genetic experiments of bean be practiced within distinct MA and Andean panels. The combination of data for two traits or environments can lead to the discovery of stronger effects than those discovered using a single marginal analysis (Korte et al.

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